Deliverables

D1.1 has the aim to define the models to be used in the course of the whole project. These will be used both to study the efficacy of the proposed therapeutic approach (luminous aerosol) and to assess its biocompatibility and possible toxicity. The chosen models for efficacy studies are characterized by an increasing complexity, starting from in vitro (bacterial species in planktonic and biofilm forms and artificial models for sputum – ASM) up to the in vivo (mouse lung infection models). Of course, all the models considered for efficacy studies share the presence of the bacterial species of P. aeruginosa and S. aureus, corresponding to the final target of the proposed therapeutic approach. The same in vitro – in vivo scheme applies to the choice of the models considered for biocompatibility/toxicity studies: in vitro: mammalian cells; multiple-species cell cultures – TCC; in vivo: the mouse model.
Being the initial choice of the bacterial species already defined, effort was devoted to the choice of the specific strains and respective infection models, based on both the analysis of literature results and the various partners’ previous know-how in their respective fields.
These same criteria were used to define the models for biocompatibility/toxicity studies.
The methodologies described in this deliverable will be revised in the light of the activities carried out during months 13-24; the final methodologies and protocols will be given in D1.3.

This report is the second deliverable of the Light4Lungs work package WP1, Selection and development of models. The Light4Lungs project isfunded by the EU’s Horizon 2020 Programme under Grant Agreement number 863102. The purpose of this deliverable is to describe the aerosol required chemo- and photo-physical properties and includes the method that will be used to obtain the action spectrum for bacterial photokilling.

This report is the second deliverable of the Light4Lungs WP7 Project Coordination & Management, and the starting version of the Data Management Plan (DMP) for the Light4Lungs project, funded by the EU’s Horizon 2020 Programme under Grant Agreement number 863102.
The purpose of the DMP is to provide an overview of all datasets collected and generated by the project and to define the Light4Lungs consortium’s associated data management policy, both at the administrative and technical levels, that is used with regard to these datasets.
The document considers and includes the structure of the Horizon 2020 DMP template1 to report on the datasets to be produced and used with a dedicated annex, as well as how it will be published and deposited.

Public site where non-confidential information regarding the project, the consortium and the outcomes will be published, as well as any actions, events and training activities will be communicated.

Chronic lung infections are among the most diffused human infections, being often associated with multidrug-resistant bacteria. In this framework, the European project “Light4Lungs” aims at synthesizing and testing an inhalable light source to control lung infections by antimicrobial photoinactivation (aPDI), addressing endogenous photosensitizers only (porphyrins) in the representative case of S. aureus and P. aeruginosa. In the search for the best emission characteristics for the aerosolized light source, this work defines and calculates the photo-killing action spectrum for lung aPDI in the exemplary case of cystic fibrosis. This was obtained by applying a semi-theoretical modelling with Monte Carlo simulations, according to previously published methodology related to stomach infections and applied to the infected trachea, bronchi, bronchioles and alveoli. In each of these regions, the two low and high oxygen concentration cases were considered to account for the variability of in vivo conditions, together with the presence of endogenous porphyrins and other relevant absorbers/diffusers inside the illuminated biofilm/mucous layer. Furthermore, an a priori method to obtain the “best illumination wavelengths” was defined, starting from maximizing porphyrin and light absorption at any depth. The obtained action spectrum is peaked at 394 nm and mostly follows porphyrin extinction coefficient behavior. This is confirmed by the results from the best illumination wavelengths, which reinforces the robustness of our approach. These results can offer important indications for the synthesis of the aerosolized light source and definition of its most effective emission spectrum, suggesting a flexible platform to be considered in further applications.